Analysis of DNA methylation in FFPE tissues using the MethyLight technology.
作者： Ashraf Dallol , Waleed Al-Ali , Amina Al-Shaibani , Fahd Al-Mulla
DOI: 10.1007/978-1-61779-055-3_13
关键词: DNA 、 Colorectal cancer 、 CpG Island Methylator Phenotype 、 Exon 、 DNA methylation 、 Methylation profiling 、 Gene 、 Laser capture microdissection 、 Biology 、 Computational biology
摘要: … as Laser microdissection from FFPE tissue followed by DNA extraction and analysis is now … with FFPE tissues. Therefore, 100–200 ng of genomic DNA extracted from FFPE tissues can …
springer.com
sci-hub.st 参考文章(13)
Cindy A. Eads, Peter W. Laird, Combined Bisulfite Restriction Analysis (COBRA) Methods of Molecular Biology. ,vol. 200, pp. 71- 86 ,(2002) , 10.1385/1-59259-182-5:071
Jean-Pierre Issa, CpG island methylator phenotype in cancer Nature Reviews Cancer. ,vol. 4, pp. 988- 993 ,(2004) , 10.1038/NRC1507
J.CIark Susan, Janet Harrison, Cheryl L. Paul, Marianne Frommer, High sensitivity mapping of methylated cytosines. Nucleic Acids Research. ,vol. 22, pp. 2990- 2997 ,(1994) , 10.1093/NAR/22.15.2990
Jean-François Gagnon, François Sanschagrin, Simon Jacob, Andrée-Anne Tremblay, Louise Provencher, Jean Robert, Carol Morin, Caroline Diorio, Quantitative DNA methylation analysis of laser capture microdissected formalin-fixed and paraffin-embedded tissues. Experimental and Molecular Pathology. ,vol. 88, pp. 184- 189 ,(2010) , 10.1016/J.YEXMP.2009.09.020
J. G. Herman, J. R. Graff, S. Myohanen, B. D. Nelkin, S. B. Baylin, Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 9821- 9826 ,(1996) , 10.1073/PNAS.93.18.9821
Shuji Ogino, Mohan Brahmandam, Takako Kawasaki, Gregory J Kirkner, Massimo Loda, Charles S Fuchs, Epigenetic profiling of synchronous colorectal neoplasias by quantitative DNA methylation analysis. Modern Pathology. ,vol. 19, pp. 1083- 1090 ,(2006) , 10.1038/MODPATHOL.3800618
Manel Esteller, Relevance of DNA methylation in the management of cancer. Lancet Oncology. ,vol. 4, pp. 351- 358 ,(2003) , 10.1016/S1470-2045(03)01115-X
Cindy A Eads, Kathleen D Danenberg, Kazuyuki Kawakami, Leonard B Saltz, Corey Blake, Darryl Shibata, Peter V Danenberg, Peter W Laird, MethyLight: a high-throughput assay to measure DNA methylation Nucleic Acids Research. ,vol. 28, pp. 32- 38 ,(2000) , 10.1093/NAR/28.8.E32
Shuji Ogino, Mohan Brahmandam, Mami Cantor, Chungdak Namgyal, Takako Kawasaki, Gregory Kirkner, Jeffrey A Meyerhardt, Massimo Loda, Charles S Fuchs, Distinct molecular features of colorectal carcinoma with signet ring cell component and colorectal carcinoma with mucinous component. Modern Pathology. ,vol. 19, pp. 59- 68 ,(2006) , 10.1038/MODPATHOL.3800482
Martin Widschwendter, Kimberly D. Siegmund, Hannes M. Müller, Heidi Fiegl, Christian Marth, Elisabeth Müller-Holzner, Peter A. Jones, Peter W. Laird, Association of Breast Cancer DNA Methylation Profiles with Hormone Receptor Status and Response to Tamoxifen Cancer Research. ,vol. 64, pp. 3807- 3813 ,(2004) , 10.1158/0008-5472.CAN-03-3852