Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA

摘要: Abstract A new method for isolating DNA from agarose gels is described. The involves the simultaneous transfer of all DNA-fragments an slab gel onto DEAE-cellulose paper and elution individual fragments with 1 M NaCl. isolated in this way susceptible to cleavage several restriction endonucleases, can be labeled vitro E coli DNA-polymerase I, T4 polynucleotide kinase. We have used construct endonuclease maps adenovirus type 16 DNA.