Antifungal activity of the essential oils from Cupressus arizonica var. arizonica and var. glabra

摘要: Abstract The composition and the evaluation of antifungal activity mechanisms action essential oils (EO) Cupressus arizonica leaves two varieties, glabra , were studied. EOs extracted by hydrodistillation chemical was determined gas chromatography/mass spectrometry (GC–MS). Both var. EOs, displayed high contents α-pinene (29.76% 26.53%, respectively) umbellulone (11.86% 15.05%, respectively). both varieties against pathogenic yeasts genus Candida investigated showed that very low concentrations EO, such as 5.10 −2  μl/ml, sufficient to inhibit growth most species, while, all except albicans (MIC = 5 × 10  μl/ml), inhibited for with only 10  μl/ml when EO used. cytotoxicity assessed in Saccharomyces cerevisiae (used a yeast experimental model) wild type mutants affected oxidative stress response DNA repair pathways. Oxidative imposed flow cytometry genotoxicity comet assay. A higher loss viability observed incubation from (5 × 10 60% loss) compared 30% . damage long tails cells exposed (17 13 μm, respectively), negative control (5 μm). Intracellular oxidation increased treated being more active oxidant activity. results obtained strain suggest cause toxicity via an mechanism. To investigate mechanism oxidation, ( yap1 ) base excision pathway apn1 investigated. show mutant strains are sensitive than type. For nucleotide rad4 ), not involved damage, similar those