Isolation, culture, and detection of arbuscular mycorrhizal fungi.

摘要: This chapter summarizes methods to (i) isolate and estimate numbers of soilborne propagules arbuscular mycorrhizal (AM) fungi, (ii) propagate AM fungi by traditional innovative methods, (iii) detect assess properties these using recent biochemical molecular technology. Soilborne may include chlamydospores or azygospores, colonized roots, hyphae. Isolates special interest should be given a unique code then classified the species level at later date. To observe structures within root, it is necessary clear cortical cells cytoplasm phenolic compounds differentially stain fungal tissue. The most commonly used obtain an total number are probable (MPN) infectivity assays. culture on plants in disinfested soil, spores, infested soil as inocula, has been frequently technique for increasing propagule numbers. Conducive environmental conditions cultures balance high light intensity, adequate moisture, moderate temperature without detrimental additions fertilizers pesticides. Biochemical have improve means detection quantification environment. Although current our ability field, monitoring abundance distribution individual remains laborious expensive.