Extremely high electric field strengths in non-aqueous capillary electrophoresis
作者： Sami Palonen , Matti Jussila , Simo P Porras , Tuulia Hyötyläinen , Marja-Liisa Riekkola
DOI: 10.1016/S0021-9673(01)00557-X
关键词: Chemistry 、 Aqueous solution 、 Analytical chemistry 、 Propanol 、 Joule heating 、 Joule effect 、 Electrolyte 、 Van Deemter equation 、 Chromatography 、 Electrophoresis 、 Capillary electrophoresis
摘要: The influence of high electric field strength on the separation basic analytes in non-aqueous alcohol background electrolyte (BGE) solutions was investigated. Increasing voltage capillary electrophoresis (CE) may be advantageous if conductivity BGE solution is low enough to allow fast separations without excessive Joule heating or band broadening. range tested 20-60 kV with methanol and ethanol, 25-60 propanol butanol as solvent for BGE. resulting strengths ranged from 660 V cm(-1) 2000 cm(-1). Experiments were made a special laboratory constructed CE instrument. efficiency vs. curve found vary solution. increase decreased case solution, but other BGEs clear maximum obtained above 30 kV. highest efficiencies achieved where reached at 45 However, reasonable even 60 extent determined by calculating temperature inside observed plate heights interpreted terms Van Deemter equation. decrease higher attributed mainly ethanol analyte adsorption wall solutions.
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