Temporal dynamics of mouse hippocampal clock gene expression support memory processing.

摘要: Hippocampal plasticity and mnemonic processing exhibit a striking time-of-day dependence likely implicate temporally structured replay of memory traces. Molecular mechanisms fulfilling the requirements sensing time capturing time-related information are coded in dynamics so-called clock genes their protein products, first discovered described hypothalamic suprachiasmatic nucleus. Using real-time PCR immunohistochemical analyses, we show that wildtype mice core components (mPer1/PER1, mPer2/PER2, mCry1/CRY1, mCry2/CRY2, mClock/CLOCK, mBmal1/BMAL1) expressed neurons all subregions hippocampus time-locked fashion over 24-h (diurnal) day/night cycle. Temporal profiling these transcriptional regulators reveals distinct parallel peaks, at times when traces usually formed and/or consolidated. The coordinated rhythmic expression hippocampal gene is greatly disordered deficient for mPer1, key player implicated both, maintenance adaptative circadian clocks. Moreover, Per1-knockout animals severely handicapped hippocampus-dependent long-term spatial learning paradigm. We propose imprint temporal structure on shape same efficacy behavioral learning.