Immunological homologues of the Arabidopsis thaliana beta 1 tubulin are polyglutamylated in Nicotiana tabacum
作者： Andrei P. Smertenko , Sara L. Lawrence , Patrick J. Hussey
DOI: 10.1007/BF01279470
关键词: Phragmoplast 、 Gene isoform 、 Molecular biology 、 Polyglutamylation 、 Biochemistry 、 Tubulin 、 Microtubule 、 Biology 、 Preprophase band 、 Nicotiana tabacum 、 Arabidopsis thaliana
摘要: Peptide-specific antibody AAB1, raised to the C-terminal 13 amino acids ofArabidopsis thaliana β1 tubulin, identifies a single electrophoretically separable β-tubulin on 2-D-gel Western blots of total protein extracts fromA. seedlings. We show that AAB1 crossreacts with two eight polyglutamylated isoforms present in purifiedNicotiana tabacum tubulin fractionated by high-resolution isoelectric focussing. Immunolocalisation studies using revealed twoN. β1-tubulin are utilised all four plant microtubule arrays (the interphase cortical array, preprophase band, spindle and phragmoplast) indicating there is no apparent subcellular sorting these isotypes.
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