The Inhibition of Human Plasmin by Human Antithrombin-Heparin Cofactor

摘要: Abstract The interaction of purified human antithrombin-heparin cofactor and plasmin was studied in the presence absence heparin. Antithrombin is a progressive, timedependent inhibitor proteolytic esterolytic activities plasmin. Incubation with antithrombin for 15 to 30 min results 90 100% inhibition both enzyme. heparin dramatically accelerates rate plasmin, nearly complete within s incubation. Sodium dodecyl sulfate gel electrophoresis reduced nonreduced proteins indicates that functions as potent antiplasmin by forming an undissociable complex which stable denaturing or reducing agents (or both). This represents 1:1 stoichiometric combination enzyme inhibitor. Heparin increases formation this without affecting its dissociability stoichiometry.