Amplification and purification of the bacteriophage Mu encoded B transposition protein.

摘要: The A and B proteins encoded by the temperate bacteriophage Mu are involved in high efficiency DNA transposition reaction which is distinguishing feature of this phage. genes encoding these early were cloned an expression vector under control lambda leftward promoter. Under optimal conditions gpB was overproduced to account for 15% total cellular protein. protein purified near homogeneity as determined silver staining. Sequence analysis N terminus confirmed identity gpB. Proteolytic processing does not occur at amino terminus; terminal methionine residue quantitatively deformylated. protein, found be basic a general binding insoluble low ionic strength absence, but presence, DNA. also displayed tendency aggregate where it soluble absence In addition, characterized its acid composition extinction coefficient 280 nm. active vitro transposition-replication system.