Potent in vitro chondrogenesis of CD105 enriched human adipose-derived stem cells

摘要: Adipose-derived stem cells (ASCs) are considered as a promising cell source for cartilage regeneration. However, the heterogeneity of this may affect their ability in formation. It is therefore necessary to establish an efficient method isolating that have chondrogenic potential. To date, no specific markers been reported be able isolate such population from human adipose tissue. In recent studies, endoglin (CD105) has known relatively marker identifying mesenchymal cells, but studies show it related potential ASCs. study, tissue were isolated, cultured, and sorted according CD105 expression. The then subjected adipogenic, osteogenic, induction confirm multi-potentiality. adipogenic conditions, CD105- showed stronger Oil Red staining higher expression adipose-specific genes compared CD105+ cells. By contrast, exhibited better osteogenic with Alizarin than Noticeably, also much collagen II gene aggrecan. Most importantly, could form homogeneous cartilage-like when seeded into biodegradable scaffold cultured media 8 weeks. These results indicate sorting ASC subpopulation allow vitro chondrogenesis thus provide important implications regeneration reconstruction using autologous