MiR-1224-5p acts as a tumor suppressor via inhibiting the malignancy of rectal cancer through targeting SLC29A3.

摘要: The emphasis of our study was to determine the physiological function miR-1224-5p in rectal cancer (RC) and its in-depth mechanism. First, expression RC tissues analyzed using public data from TCGA database. Then, cell lines SW480 SW837 measured qRT-PCR assay. subsequent CCK-8 assay executed assess viability cell. Bioinformatics prediction prompted that SLC29A3 may be a potential target gene for miR-1224-5p. Western blotting dual-luciferase reporter assays were performed affirm above forecasting. Kaplan-Meier analysis Cox multivariate carried out relationship between prognosis. Finally, CCK-8, colony formation assay, transwell used functional miR-1224-5p/ axis vitro. MiR-1224-5p expressed at low levels lines. Up-regulation inhibited viability, while inhibition enhanced cells. What is more, we affirmed could direct SLC29A3, which high tissues. In addition, as an independent predictive factor prognosis patients with RC, higher expression, lower survival rate. cellular experiments evidenced mimic can reduce invasion, migration, overexpression presented opposite effect. Importantly, co-transfection indicated reverse miR-1224-5p-mediated malignant progression Our work raised possibility functioned tumor suppressor achieved via targeting SLC29A3.