Carbohydrate-binding modules: fine-tuning polysaccharide recognition

摘要: The enzymic degradation of insoluble polysaccharides is one the most important reactions on earth. Despite this, glycoside hydrolases attack such relatively inefficiently as their target glycosidic bonds are often inaccessible to active site appropriate enzymes. In order overcome these problems, many that utilize substrates modular, comprising catalytic modules appended or more non-catalytic CBMs (carbohydrate-binding modules). promote association enzyme with substrate. view central role play in hydrolysis plant structural and storage polysaccharides, ligand specificity displayed by protein mechanism which they recognize carbohydrates have received considerable attention since discovery almost 20 years ago. last few years, CBM research has harnessed structural, functional bioinformatic approaches elucidate molecular determinants drive CBM–carbohydrate recognition. present review summarizes impact biology had our understanding mechanisms bind ligands.