作者: Osipov An , Pustovalova Mv , Smetanina Nm
DOI:
关键词: DNA 、 Alkali metal 、 DNA damage 、 Nucleoid 、 Lymphocyte 、 Biology 、 Molecular biology 、 Dimethyl sulfoxide 、 Radiation 、 Radical
摘要: It is shown that exposure of 365 nm UV radiation at doses 10, 20 and 50 kJ/m2 induces a dose-dependent increase in DNA single-strand breaks alkali-labile sites (SSB ALS) detected by comet halo assays human blood lymphocyte nucleoids. Adding 10% dimethyl sulfoxide (DMSO) reduces the SSB ALS yields--in 3 times. A strong drop output UV-A-induced nucleoids presence DMSO shows leading role *OH radicals this damage formation under to 365-nm UV-radiation.