Gut segments outweigh the diet in shaping the intestinal microbiome composition in grass carp Ctenopharyngodon idellus

摘要: Although dynamics of the complex microbial ecosystem populating gastrointestinal tract animals has profound and multifaceted impacts on host's metabolism health, it remains unclear whether is intrinsic or extrinsic factors that play a more dominant role in mediating variations composition intestinal microbiota. To address this, two strikingly different diets were studied: high-protein, low-fiber formula feed (FF), low-protein, high-fiber Sudan grass (SG). After 16-week feeding trial herbivorous fish, carp, profiles midgut (M) hindgut (H) segments both groups compared. Bacteroidetes abundant (T=-7.246, p<0.001), Proteobacteria (T=4.383, p<0.001). Fusobacteria FF group (compared to SG group, T=2.927, Bacterial was (p<0.05) between midguts (M-FF) (M-SG) groups, but not hindguts (H-FF H-SG; p=0.269). PerMANOVA VPA indicated gut segment contributed 19.8% (p<0.001) 28% variation communities, whereas diet only 8.0% 14% (p<0.001), respectively. Overall, results suggest compartments are stronger determinant than shaping Specifically, strong impact microbiome proximal compartments, this much less pronounced distally, which likely be reflection limited ability some taxa thrive anoxic environment distal segments.