Secretome of Aggregated Embryonic Stem Cell-Derived Mesenchymal Stem Cell Modulates the Release of Inflammatory Factors in Lipopolysaccharide-Induced Peripheral Blood Mononuclear Cells
作者： Nastaran Mohammadi Ghahhari , Faezeh Maghsood , Saeed Jahandideh , Majid Lotfinia , Shirin Lak
关键词: Cell biology 、 Embryonic stem cell 、 Chemistry 、 Peripheral blood mononuclear cell 、 Mesenchymal stem cell 、 Secretion 、 Inflammation 、 Cell aggregation 、 Immunophenotyping 、 Lipopolysaccharide
摘要: Background Bone marrow mesenchymal stem cells (BM-MSCs) have emerged as a potential therapy for various inflammatory diseases. Because of some limitations, several recent studies suggested the use embryonic cell-derived MSCs (ESC-MSCs) an alternative BM-MSCs. Some therapeutic effects ESC-MSCs are related to secretion broad array cytokines and growth factors, known secretome. Harnessing this secretome applications requires optimization production secretary molecules. It has been shown that aggregation into 3D spheroids, preconditioning strategy, can enhance immunomodulatory such cells. In study, we investigated effect derived from human (hESC-MSCs) spheroids on IL-1β, IL-10, tumor necrosis factor α (TNF-α) lipopolysaccharide (LPS)-induced peripheral blood mononuclear (PBMCs). Methods present after immunophenotyping considering mesodermal differentiation hESC-MSCs, were non-adherently grown prepare aggregates, then conditioned medium or was extracted cultures. Afterwards, anti-inflammatory assessed in vitro model inflammation. Results study showed aggregate-prepared hESC-MSCs able significantly decrease TNF-α (301.7 ± 5.906, p < 0.0001) IL-1β (485.2 48.38, 0.001) LPS-induced PBMCs indicators inflammation, comparison with adherent culture-prepared (TNF-α: 166.6 8.04, IL-1β: 125.2 2.73). Conclusion Our indicated cell be appropriate strategy increase characteristics hESC-MSCs.
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