Electrokinetically controlled real-time polymerase chain reaction in microchannel using Joule heating effect
作者： Guoqing Hu , Qing Xiang , Rachel Fu , Bo Xu , Roberto Venditti
DOI: 10.1016/J.ACA.2005.10.021
关键词: Work (thermodynamics) 、 TaqMan 、 Microchannel 、 Joule heating 、 Joule effect 、 Microfluidics 、 Joule 、 Analytical chemistry 、 Temperature cycling 、 Chemistry 、 Optoelectronics 、 Spectroscopy 、 Biochemistry 、 Environmental chemistry
摘要: In this work, a new method to control polymerase chain reaction (PCR) thermal cycling in microchannel using Joule heating effect was presented. generated internally by the current flow through buffer solution an electrokinetically-driven microfluidic chip without external heater component. Numerical simulations were developed and conducted determine parameters required achieve desired cycling. A PDMS-based PCR fabricated tested. The power consumption only 1.3 W for such heating-controlled chip. To demonstrate feasibility of cycling, DNA fragment Escherichia coli O157:H7 stx1 successfully amplified two-temperature TaqMan real-time reaction.
elsevier.com
sci-hub.se 参考文章(36)
Dae-Sik Lee, Se Ho Park, Haesik Yang, Kwang-Hyo Chung, Tae Hwan Yoon, Sung-Jin Kim, Kyuwon Kim, Youn Tae Kim, Bulk-micromachined submicroliter-volume PCR chip with very rapid thermal response and low power consumptionElectronic supplementary information (ESI) available: details of numerical simulation. See http://www.rsc.org/suppdata/lc/b3/b313547k/ Lab on a Chip. ,vol. 4, pp. 401- 407 ,(2004) , 10.1039/B313547K
Kai Sun, Akira Yamaguchi, Yutaka Ishida, Shigeki Matsuo, Hiroaki Misawa, A heater-integrated transparent microchannel chip for continuous-flow PCR Sensors and Actuators B-chemical. ,vol. 84, pp. 283- 289 ,(2002) , 10.1016/S0925-4005(02)00016-3
Jian Liu, Markus Enzelberger, Stephen Quake, None, A nanoliter rotary device for polymerase chain reaction. Electrophoresis. ,vol. 23, pp. 1531- 1536 ,(2002) , 10.1002/1522-2683(200205)23:10<1531::AID-ELPS1531>3.0.CO;2-D
Frank P. Incropera, David P. DeWitt, Introduction to Heat Transfer ,(1985)
D. J. Harrison, K. Fluri, K. Seiler, Z. Fan, C. S. Effenhauser, A. Manz, Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip Science. ,vol. 261, pp. 895- 897 ,(1993) , 10.1126/SCIENCE.261.5123.895
M. A. Strausbauch, A. F. R. Huhmer, N. Borson, S. R. Jurrens, J. Craighead, P. J. Wettstein, B. Eckloff, B. Kline, J. P. Landers, R. P. Oda, Infrared-Mediated Thermocycling for Ultrafast Polymerase Chain Reaction Amplification of DNA Analytical Chemistry. ,vol. 70, pp. 4361- 4368 ,(1998) , 10.1021/AC980452I
Larry C. Waters, Stephen C. Jacobson, Natalia Kroutchinina, Julia Khandurina, Robert S. Foote, J. Michael Ramsey, Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip Analytical Chemistry. ,vol. 70, pp. 5172- 5176 ,(1998) , 10.1021/AC980447E
Carl T. Wittwer, G.Chris Fillmore, David J. Garling, Minimizing the time required for DNA amplification by efficient heat transfer to small samples Analytical Biochemistry. ,vol. 186, pp. 328- 331 ,(1990) , 10.1016/0003-2697(90)90090-V
Karen C. Jinneman, Ken J. Yoshitomi, Stephen D. Weagant, Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H− Serotype Applied and Environmental Microbiology. ,vol. 69, pp. 6327- 6333 ,(2003) , 10.1128/AEM.69.10.6327-6333.2003
Kelly Swinney, Darryl J. Bornhop, Quantification and evaluation of Joule heating in on-chip capillary electrophoresis. Electrophoresis. ,vol. 23, pp. 613- 620 ,(2002) , 10.1002/1522-2683(200202)23:4<613::AID-ELPS613>3.0.CO;2-F