Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections.
作者： Sascha Al Dahouk , Karsten Nöckler , Holger C. Scholz , Herbert Tomaso , Ralf Bogumil
DOI: 10.1016/J.JIM.2005.11.003
关键词: Brucella 、 GroEL 、 Microbiology 、 Cross-reactivity 、 Brucella melitensis 、 Antibody 、 Brucellaceae 、 Biology 、 Agglutination (biology) 、 Virology 、 Antigen
摘要: The diagnosis of brucellosis is mainly based on the detection anti-LPS antibodies. Due to substantial similarity O-polysaccharide Brucella LPS that various other Gram-negative bacteria, serological tests samples containing high amounts lack specificity. Hence, development assays more specific protein antigens an essential subject in research. aim this study was proteomic characterization antigen preparations diagnostic reference strain abortus 1119-3 and identification immunogenic proteins suitable for assays. Seventeen out 383 spots B. were identified be by 2-D immunoblotting. These assigned 6 MALDI-MS nLC-ESI-MS/MS: Cu-Zn SOD, BCSP31, L7/L12, GroEL, GroES, DnaK. All present three different investigated, i.e. native antigen, standard agglutination commercially available antigen. immunoblotting bacteria cross-reacting with Brucellae proved cross-reactivity negligible. Surface enhanced laser desorption/ionization mass spectrometry (SELDI-MS) spectra also differentiated clearly from bacteria. combination SELDWS analysis specificity antibody binding will improve proteins. (c) 2005 Elsevier B.V. rights reserved
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