Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)
作者： G. Nagarajan , Shelesh Kumar Swami , S.K. Ghorui , K.M.L. Pathak , R.K. Singh
DOI: 10.1016/J.RVSC.2011.03.028
关键词: Peptide sequence 、 Phylogenetic tree 、 Amino acid 、 Biology 、 Cloning 、 Bactrian camel 、 Molecular biology 、 Phylogenetics 、 Sequence analysis 、 Nucleotide
摘要: The cDNAs of three cytokines, viz., IL-2, IL-4 and IFN-γ from Dromedary camels were amplified by PCR using Bactrian camel sequences subsequently cloned for sequence analysis. Relationship based on amino acid revealed that IL-2 shared 99.5% 99.3% identity at the nucleotide levels with IL-2. In case IL-4, was 99.7% 99.2% levels, respectively camel. 100% both IFN-γ. Phylogenetic analysis indicated close relationship in these cytokine genes between other camelids.
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