Rapid Colorimetric Detection of Pseudomonas aeruginosa in Clinical Isolates Using a Magnetic Nanoparticle Biosensor
作者： Sahar Alhogail , Ghadeer A.R.Y. Suaifan , Floris J. Bikker , Wendy E. Kaman , Karina Weber
关键词: Naked eye 、 Biosensor 、 Substrate (chemistry) 、 Magnetic nanoparticles 、 Biochip 、 Detection limit 、 Chemistry 、 Nanoparticle 、 Pseudomonas aeruginosa 、 Chromatography
摘要: A rapid, sensitive, and specific colorimetric biosensor based on the use of magnetic nanoparticles (MNPs) was designed for detection Pseudomonas aeruginosa in clinical samples. The biosensing platform measurement P. proteolytic activity using a protease substrate. At N-terminus, this substrate covalently bound to MNPs linked gold sensor surface via cystine at C-terminus substrates. golden appears black naked eyes because coverage MNPs. However, upon proteolysis, cleaved peptide-MNP moieties will be attracted by an external magnet, revealing color surface, which can observed eye. In vitro, able detect specifically quantitatively presence with limit 102 cfu/mL less than 1 min. used test its ability situ isolates from patients. This biochip is anticipated useful as rapid point-of-care device diagnosis aeruginosa-related infections.
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