Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission.

摘要: A nested polymerase chain reaction (PCR) protocol was developed for rapid genotyping of hepatitis B virus (HBV). During the first PCR round, a universal HBV primer pair used to amplify entire pre-S region genome. Within region, many nucleotide exchanges are observed. These partly correlated serological surface antigen subtypes. Five additional subtype-specific primers were selected from that which, together with two non-group-specific primers, generated specific combinations four DNA fragments defined sizes. By this approach, 55 antigen-positive patients pediatric oncology unit in Germany analyzed. Fifty-four who had been infected within 2 years an identical pattern multiplex PCR, suggesting common source infection and person-to-person transmission unit. One child 5 later different and, therefore, must have source. Furthermore, 109 serum samples taken pregnant Cameroonian women 25 their babies 6 months after birth In one case, mother-to-infant demonstrated. Apart its role epidemiological studies on HBV, may also be useful tool genetic analysis other fields if there is moderate degree sequence variation which enables design primers.