G-Protein β3-Subunit 825T Allele Is Associated With Enhanced Human Atrial Inward Rectifier Potassium Currents
作者： D. Dobrev , E. Wettwer , H. M. Himmel , A. Kortner , E. Kuhlisch
关键词: Endocrinology 、 G protein 、 Heterotrimeric G protein 、 Inward-rectifier potassium ion channel 、 Genotype 、 Potassium channel 、 Depolarization 、 Myocyte 、 Internal medicine 、 Adenosine 、 Biology
摘要: Background—A C825T polymorphism was recently identified in the human gene encoding for β3-subunit of heterotrimeric G proteins. The 825T allele is associated with a splice variant Gβ3 and enhanced signal transduction. We hypothesized that patients carrying exhibit modified phenotype. resulting enhancement transduction should be detectable Gβγ-dimer–mediated acetylcholine-stimulated K+ current (IK,ACh). Methods Results—Seventy undergoing cardiac surgery were genotyped polymorphism. In right atrial myocytes from these patients, inward rectifier currents (IK1, IK,ACh) studied whole-cell patch-clamp technique. Background IK1 measured depolarizing ramp pulses quantified as at −100 mV; mean amplitudes (pA/pF) 4.98±0.49 (n=30/93 patients/cells) CC genotype, 4.25±0.36 (n=31/121 TC, 7.46±1.14 (n=9/32 patients/cells; P<0.05) TT. C...
sci-hub.se 参考文章(19)
Taroh Iiri, Zvi Farfel, Henry R. Bourne, G-protein diseases furnish a model for the turn-on switch Nature. ,vol. 394, pp. 35- 38 ,(1998) , 10.1038/27831
Grigory Krapivinsky, Luba Krapivinsky, Kevin Wickman, David E. Clapham, G beta gamma binds directly to the G protein-gated K+ channel, IKACh. Journal of Biological Chemistry. ,vol. 270, pp. 29059- 29062 ,(1995) , 10.1074/JBC.270.49.29059
David R. Van Wagoner, Amber L. Pond, Patrick M. McCarthy, James S. Trimmer, Jeanne M. Nerbonne, Outward K+ Current Densities and Kv1.5 Expression Are Reduced in Chronic Human Atrial Fibrillation Circulation Research. ,vol. 80, pp. 772- 781 ,(1997) , 10.1161/01.RES.80.6.772
C Kleuss, H Scherubl, J Hescheler, G Schultz, B Wittig, Selectivity in signal transduction determined by gamma subunits of heterotrimeric G proteins Science. ,vol. 259, pp. 832- 834 ,(1993) , 10.1126/SCIENCE.8094261
Peter H. Barry, JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements Journal of Neuroscience Methods. ,vol. 51, pp. 107- 116 ,(1994) , 10.1016/0165-0270(94)90031-0
S Koumi, C E Arentzen, C L Backer, J A Wasserstrom, Alterations in muscarinic K+ channel response to acetylcholine and to G protein-mediated activation in atrial myocytes isolated from failing human hearts. Circulation. ,vol. 90, pp. 2213- 2224 ,(1994) , 10.1161/01.CIR.90.5.2213
D. Rosskopf, K.-J. Schroder, W. Siffert, Role of sodium-hydrogen exchange in the proliferation of immortalised lymphoblasts from patients with essential hypertension and normotensive subjects Cardiovascular Research. ,vol. 29, pp. 254- 259 ,(1995) , 10.1016/S0008-6363(96)88578-6
G J Amos, E Wettwer, F Metzger, Q Li, H M Himmel, U Ravens, Differences between outward currents of human atrial and subepicardial ventricular myocytes. The Journal of Physiology. ,vol. 491, pp. 31- 50 ,(1996) , 10.1113/JPHYSIOL.1996.SP021194
Winfried Siffert, Dieter Rosskopf, Gerlinde Siffert, Stefan Busch, Albrecht Moritz, Raimund Erbel, Arya M. Sharma, Eberhard Ritz, H.-Erich Wichmann, Karl H. Jakobs, Bernhard Horsthemke, Association of a human G-protein β3 subunit variant with hypertension Nature Genetics. ,vol. 18, pp. 45- 48 ,(1998) , 10.1038/NG0198-45
Zhiguo Wang, Lixia Yue, Michel White, Guy Pelletier, Stanley Nattel, Differential Distribution of Inward Rectifier Potassium Channel Transcripts in Human Atrium Versus Ventricle Circulation. ,vol. 98, pp. 2422- 2428 ,(1998) , 10.1161/01.CIR.98.22.2422