Strongylus asini (Nematoda, Strongyloidea): Genetic relationships with other strongylus species determined by ribosomal DNA
作者： Guo-Chiuan Hung , Dennis E. Jacobs , Rosina C. Krecek , Robin B. Gasser , Neil B. Chilton
DOI: 10.1016/S0020-7519(96)00136-1
关键词: Ribosomal RNA 、 Biology 、 Strongyloidea 、 Ribosomal DNA 、 Genetics 、 Restriction fragment length polymorphism 、 Polymerase chain reaction 、 Strongylus vulgaris 、 Strongylus 、 Molecular biology 、 genomic DNA
摘要: Genomic DNA was isolated from adult Strongylus asini collected zebra. The second ribosomal transcribed spacer (ITS-2) amplified and sequenced using polymerase chain reaction (PCR) based techniques. sequence compared with previously published data for 3 related species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. ITS-2 of S. found more similar those edentatus (87.1%) equinus (95.3%) than that S vulgaris (73.9%). This result confirms Asini represent separate supports retention within 1 genus.
elsevier.com
sci-hub.se 参考文章(11)
K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich, Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction Cold Spring Harbor Symposia on Quantitative Biology. ,vol. 51, pp. 263- 273 ,(1986) , 10.1101/SQB.1986.051.01.032
Lynda M. Gibbons, An SEM study of the cephalic region, buccal cavity and male tail of the species of the genus Strongylus Müller, 1780 (Nematoda, Strongyloidea). Journal of Helminthology. ,vol. 58, pp. 149- 158 ,(1984) , 10.1017/S0022149X00028686
Charles L. Boulenger, Sclerostomes of the Donkey in Zanzibar and East Africa Parasitology. ,vol. 12, pp. 27- 32 ,(1920) , 10.1017/S0031182000013986
F.S. Malan, Rosina C. Scialdo-Krecek, H.J. Els, Scanning electron microscopy of Strongylus spp. in zebra. Onderstepoort Journal of Veterinary Research. ,vol. 50, pp. 257- ,(1983)
J.M. Pletcher, F.S. Malan, Valerius De Vos, R.K. Reinecke, Studies on Strongylus asini. I. Experimental infestation of equines. Onderstepoort Journal of Veterinary Research. ,vol. 49, pp. 151- ,(1982)
J. R. Lichtenfels, Keys to genera of the superfamily Strongyloidea. Keys to genera of the superfamily Strongyloidea.. ,(1980)
K. I. Skri︠a︡bin, Gelʹmintologicheskai︠a︡ laboratorii︠a︡, Key to parasitic nematodes Israel Program for Scientific Translations. ,(1961)
Robert R. Sokal, Peter H. A. Sneath, Numerical Taxonomy: The Principles and Practice of Numerical Classification ,(1973)
Angus J.D. Campbell, Robin B Gasser, Neil B. Chilton, Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs International Journal for Parasitology. ,vol. 25, pp. 359- 365 ,(1995) , 10.1016/0020-7519(94)00116-6
R.B. Gasser, H. Hoste, Genetic markers for closely-related parasitic nematodes Molecular and Cellular Probes. ,vol. 9, pp. 315- 319 ,(1995) , 10.1016/S0890-8508(95)91588-5