Comparison of the MagNA pure LC automated system and the RiboPure-Blood RNA manual method for RNA extraction from multiple myeloma bone marrow samples conserved in an RNA stabilizer.
作者： G. GARCIA-EFFRON , S. GAMARRA , A. CROOKE , P. MARTÍNEZ-SÁNCHEZ , J. LAHUERTA
DOI: 10.1111/J.1751-553X.2006.00830.X
关键词: RNA 、 Bone marrow 、 Extraction methods 、 Extraction (chemistry) 、 Multiple myeloma 、 Biology 、 RNA extraction 、 Research purpose 、 Molecular biology
摘要: A total of 62 frozen bone marrow specimens conserved in RNA later (Ambion) were processed using two different extraction methods, the MagNA Pure LC system (MAG; Roche) and manual RiboPure-Blood method (RIBO; Ambion); Beta glucoronidase (GUS) was amplified by LightCycler PCR to evaluate quality both procedures. Less than 1000 GUS copies/ml detected 26 (41.94%) MAG five (8.06%) RIBO. Moreover, recovery from is, on average, 2.91 cycle threshold-fold higher RIBO (P = 0.0008). Furthermore, we compared times reagent costs methods. In conclusion, is faster process 32 samples less expensive but it not sensitive enough be employed for research purpose our laboratory.
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