HER-2 fluorescence in situ hybridization: results from the survey program of the College of American Pathologists.

摘要: Abstract Context.—Fluorescence in situ hybridization (FISH) is a common method used to determine HER-2 status breast cancer. Limited information available concerning reproducibility of FISH determining gene amplification. Objective.—To present proficiency testing results for conducted by the Cytogenetics Resource Committee College American Pathologists/American Medical Genetics. Design.—During past 5 years, unstained sections from 9 invasive carcinomas were testing, allowing comparison among large number laboratories. Additional data collected using an educational (ungraded) challenge and supplemental questions surveys. Results.—The laboratories participating has increased steadily during years (from 35 2000 139 2004). Reproducibility test was excellent tumors with low copy ...