Zinc up‐regulates NF‐κB activation via phosphorylation of IκB in HUT‐78 (Th0) cells
作者： Bin Bao , Ananda S Prasad , Frances WJ Beck , Fazlul H Sarkar , None
DOI: 10.1016/J.FEBSLET.2007.08.030
关键词: Chemistry 、 Biochemistry 、 Wild type 、 NF-κB 、 Transfection 、 Gene expression 、 IκB kinase 、 Transcription factor 、 Cell culture 、 Phosphorylation 、 Cell biology
摘要: Nuclear factor of kappaB (NF-kappaB) is a major transcription regulating the expression interleukin-2 (IL-2) and receptor-alpha (IL-2Ralpha) in Th(1) cells. We previously demonstrated that zinc increased IL-2 IL-2Ralpha production via NF-kappaB activation HUT-78 (Th(0)) However, molecular mechanism not well understood. In this study, we found phosphorylated IkappaB-alpha, translocation activation, as wild type IkappaB gene transfected zinc-sufficient cells, compared to zinc-deficient dominant negative decreased these parameters suggesting pathway.
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sci-hub.se 参考文章(11)
Peter J. Barnes, Nuclear factor-kappa B. The International Journal of Biochemistry & Cell Biology. ,vol. 29, pp. 867- 870 ,(1997) , 10.1016/S1357-2725(96)00159-8
Ananda S. Prasad, Biochemistry of zinc ,(1993)
U Zabel, R Schreck, P A Baeuerle, DNA binding of purified transcription factor NF-kappa B. Affinity, specificity, Zn2+ dependence, and differential half-site recognition. Journal of Biological Chemistry. ,vol. 266, pp. 252- 260 ,(1991) , 10.1016/S0021-9258(18)52428-5
A H Shankar, A S Prasad, Zinc and immune function: the biological basis of altered resistance to infection. The American Journal of Clinical Nutrition. ,vol. 68, pp. 447- 463 ,(1998) , 10.1093/AJCN/68.2.447S
Ananda S Prasad, Bin Bao, Frances WJ Beck, Fazlul H Sarkar, None, Zinc enhances the expression of interleukin-2 and interleukin-2 receptors in HUT-78 cells by way of NF-κB activation Journal of Laboratory and Clinical Medicine. ,vol. 140, pp. 272- 289 ,(2002) , 10.1067/MLC.2002.127908
Bin Bao, Ananda Prasad, Frances WJ Beck, Anupam Suneja, Fazlul Sarkar, None, Toxic effect of zinc on NF-κB, IL-2, IL-2 receptor α, and TNF-α in HUT-78 (Th0) cells Toxicology Letters. ,vol. 166, pp. 222- 228 ,(2006) , 10.1016/J.TOXLET.2006.07.306
Masami Otsuka, Mikako Fujita, Tsutomu Aoki, Shunsuke Ishii, Yukio Sugiura, Tadashi Yamamoto, Jun-ichiro Inoue, Novel Zinc Chelators with Dual Activity in the Inhibition of the .kappa.B Site-Binding Proteins, HIV-EP1 and NF-.kappa.B Journal of Medicinal Chemistry. ,vol. 38, pp. 3264- 3270 ,(1995) , 10.1021/JM00017A011
Ananda S Prasad, Bin Bao, Frances WJ Beck, Fazlul H Sarkar, None, Correction of interleukin-2 gene expression by in vitro zinc addition to mononuclear cells from zinc-deficient human subjects: A specific test for zinc deficiency in humans Translational Research. ,vol. 148, pp. 325- 333 ,(2006) , 10.1016/J.TRSL.2006.07.008
Ananda S Prasad, Bin Bao, Frances WJ Beck, Fazlul H Sarkar, None, Zinc activates NF-κB in HUT-78 cells Journal of Laboratory and Clinical Medicine. ,vol. 138, pp. 250- 256 ,(2001) , 10.1067/MLC.2001.118108
Ulrich Siebenlist, Guido Franzoso, Keith Brown, Structure, regulation and function of NF-kappa B. Annual Review of Cell Biology. ,vol. 10, pp. 405- 455 ,(1994) , 10.1146/ANNUREV.CB.10.110194.002201