Seroprevalence of Neospora caninum in non-carnivorous wildlife from Spain

摘要: Serum samples from 1034 non-carnivorous wildlife Spain were tested for antibodies to Neospora caninum by competitive screening enzyme linked immunosorbent assay (ELISA) and confirmed an indirect fluorescent antibody test (IFAT). High agreement was observed between results in both techniques (kappa value higher than 0.9). Prevalences of N. positive 11.8% 237 red deer (Cervus elaphus), 7.7% 13 barbary sheep (Ammotragus lervia), 6.1% 33 roe (Capreolus capreolus) 0.3% 298 wild boar (Sus scrofa). In one 53 hares (Lepus granatensis), found the ELISA but could not be IFAT due lack sample. Antibodies any 251 rabbits (Oryctolagus cuniculus), 79 fallow (Dama dama), 27 mouflon (Ovis ammon), 40 chamois (Rupicapra pyrenaica) three Spanish ibex (Capra pyrenaica). Statistically significant differences seroprevalence management hunting estates (open versus fenced) with prevalence fenced estates, among sampling sites. Seroprevalence particularly high some areas (MO estate South-Central or Catalonia, North-East Spain), while no contact others. Results indicate that certain Spain, is present wildlife, especially deer. These have important implications sylvatic cycles may influence infection cattle farms those areas. To our knowledge, this first report boar.