Ethanolamine utilization in Salmonella typhimurium.

摘要: Ethanolamine can serve as the sole source of carbon and nitrogen for Salmonella typhimurium if vitamin B12 is present to a cofactor. The pathway ethanolamine utilization has been investigated in order understand its regulation determine whether important selective forces that have maintained ability synthesize S. typhimurium. We isolated mutants are defective (eut mutants). These defined cluster genes located between purC cysA at 50 min on chromosome. A genetic map eut region was constructed. Included mutations which affect ammonia lyase, first degradative enzyme, second enzyme pathway, acetaldehyde dehydrogenase. Transcriptional studied by using eut-lac operon fusions created insertion Mu d lac. Transcription induced simultaneous presence growth medium. constitute single unit transcription. One class promoter-distal end prevent induction