Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection
作者： K. Dalal , B. Dalal , S. Bhatia , A. Shukla , A. Shankarkumar
DOI: 10.1016/J.CCA.2019.04.072
关键词: Lectin 、 Cirrhosis 、 Immunoassay 、 Hepatitis B virus 、 Immunology 、 Glycoproteomics 、 Hepatocellular carcinoma 、 Haptoglobin 、 Medicine 、 Fucosylation
摘要: Abstract Background Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries. Its early detection of paramount importance using marker having both sensitivity and specificity. The present study promises diagnostic prognostic markers by the identification site-specific glycoforms on Haptoglobin (Hp) LC-MS/MS lectin ELISA liver diseased conditions HBV infection. Methods Three groups patients: chronic, cirrhosis HCC with along controls were enrolled. Hp was purified affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition identified mass spectrometry. Quantitative used measure levels fucosylation diseases HBV. Results significantly lower when compared Non-HCC cases (p Conclusion Quantitation protein Lectin may be useful glycobiomarker either alone or combination (AFP + DCP + FucHp; AUC = 0.94) diagnosis clinical practice.
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