Effects of a protecting osmolyte on the ion atmosphere surrounding DNA duplexes.

摘要: Osmolytes are small, chemically diverse, organic solutes that function as an essential component of cellular stress response. Protecting osmolytes enhance protein stability via preferential exclusion, and nonprotecting osmolytes, such urea, destabilize structures. Although much is known about osmolyte effects on proteins, less understood nucleic acids their counterion atmospheres. Nonprotecting acid structures, but protecting depend numerous factors including the type complexity functional fold. To begin quantifying interactions, we used small-angle X-ray scattering (SAXS) techniques to monitor DNA duplexes in presence sucrose. This a commonly contrast matching agent SAXS studies protein–nucleic complexes; thus, it important characterize interaction changes induced by Measure...