Living related conjunctival limbal allograft for the treatment of stem cell deficiency

摘要: Abstract Purpose To evaluate the outcomes of living related conjunctival limbal allograft transplantation for treatment stem cell deficiency. Design Retrospective, noncomparative case series. Participants Nine donors, eight recipients (10 eyes) with Stevens-Johnson syndrome (3 eyes), ectodermal dysplasia chemical injury (2 ocular cicatricial pemphigoid (1 eye), and atopic keratoconjunctivitis (n = 1). Intervention Four clock hours tissue from best matched human leukocyte antigen (HLA) relative donor were transplanted to recipient eye superiorly inferiorly after peritomy removal pannus. Systemic cyclosporine was administered all recipients. Main outcome measures Restoration corneal epithelium, reduction vascularity conjunctivalization, improved comfort, clarity, visual improvement. Results Mean follow-up period 26.2 months. Two highly inflamed eyes failed initially epithelialize. The remainder survived restoration epithelium vascularization. Corneal opacification reduced (four improvement achieved in seven eyes. All five pain had an symptoms. Allograft rejection occurred two (25%), both treated successfully. Both class I HLA mismatches, underlying diagnosis syndrome. One developed a recurrent epithelial defect perforated, requiring penetrating keratoplasty that remained clear intact surface. initial failures also perforated required keratoplasties failed. None any complications. Conclusions surface by HLA-matched can be accomplished selected cyclosporine, even at low doses, is useful ensuring long-term survival.