Comparative attempts for the establishment and optimisation of a PCR on Leishmania for the purpose of diagnosis

摘要: Leishmania spp. are the causative agents of visceral, cutaneous and mucocutaneous leishmaniosis with several taxa ("species") genus being involved in human disease. As diagnostics based on microscopical detection parasites or serological tests often unsatisfactory, also molecular biological methods, particularly polymerase chain reaction (PCR), have been employed for past years. The aim present study was to compare different PCR-protocols optimise them our needs, placing emphasis improvement DNA isolation. PCR performed whole cell isolated from cultures, as well simulated blood samples clinical samples. Three methods isolation two PCR-protocols, one amplifying a fragment 18S rDNA amplification kDNA-circle, were applied compared. No significant difference sensitivity detected between however, it shown that highest yield achieved protocol urea.