Chapter 7 Patch Clamp of Cation Channels

摘要: Publisher Summary Recently, the patch-clamp technique has been used to study ion transport in epithelial tissues. The ability of an tissue perform net is a consequence asymmetric distribution channels and transporters apical basolateral membranes cells. Application techniques epithelia complicated by this assymmetric channel populations. This chapter discusses recent advances application cation tissue. It defines as proteinaccous pore that spans lipid bilayer, allows cations flow through membrane-spanning protein opposed bilayer. Indeed, would be adequate definition if could not permeate anion-selective channels. reviews technical approaches investigators epithelia. also outlines experimental physiological relevance data obtained from three different tissues are discussed.