Different histological types of non-small cell lung cancer have distinct folate and DNA methylation levels.

摘要: Aberrant DNA methylation is a commonly observed epigenetic change in lung cancer. Folate has been suggested to play role the homeostasis of and also implicated cancer chemotherapy. We investigated possible for folate by measuring concentrations tumors adjacent normal tissues from 72 non-small cell (NSCLC) patients. These were compared levels clinicopathological features. determined as sum 5,10-methylenetetrahydrofolate tetrahydrofolate. The MethyLight assay was used quantitate promoter regions P16(CDKN2A), APC, CDH13, RARB, RASSF1, RUNX3, MYOD1. Methylation LINE-1 repeats surrogate global methylation. correlated positively with LINE-1, RUNX3 significantly higher adenocarcinoma squamous carcinoma (SCC). Two sets array-based data retrieved Gene Expression Omnibus consistently showed that expression FOLR1, transport enzyme, GGH, an enzyme prevents retention, lower, respectively, adenocarcinomas SCC. This independently validated quantitative RT-PCR 26 13 Our results suggest metabolism plays aberrant NSCLC. histological subtype differences concentration here associated distinct patterns metabolizing enzymes. findings may have clinical applications histology-directed chemotherapy fluoropyrimidine anti-folates