Micropropagation of Stevia rebaudiana Through Leaf Explants from Adult Plants.

摘要: A micropropagation method for STEVIA REBAUDIANA plants was established by culturing young leaves of adult on medium containing 6-benzyladenine (BA) (2.0 mg/l) in the light or BA plus 1-naphthaleneacetic acid (NAA) dark. An open-ended system obtained transfer regenerating material to (0.1 mg/l), with continuous subculture and isolation suitable shoots. Auxin addition rooting (especially at 0.1 mg/l 3-indolebutyric - IBA) favored root formation improved EXTRA VITRUM plant survival. Regenerated transferred soil were uniform cytological observation tip cells showed normal chromosome number (2n = 22). Cultures treated colchicine originated altered plants.