ME-14PRO-MIGRATORY AND -PROLIFERATIVE EFFECTS OF ASTROCYTES ON GBM CELLS

摘要: Glioblastoma multiforme (GBM) is among the most lethal types of cancer. The pathological tissue contains different tumor-associated cell types, including astrocytes, which contribute to cancer biology. Our work aims at exploring potential role(s) crosstalk between astrocytes and malignant cells in GBM with regard invasion, growth drug response cells. Studies are performed double intention identifying molecular details such pathogenic paracrine cross-talk establish high-throughput assays for identification novel candidate therapeutic agents. In order look astrocyte-dependent effects on glioblastoma proliferation migration, we have co-culture experiments, direct co-culture, transwell conditioned medium assays. Interestingly, identified consistent stimulatory migration a system where an initial amount 1000 seeded or without grow much more than monoculture. assay some clearly increase their upon indirect contact (U87, A172, HF66), whereas others minimally affected by co-culture. A172 HF66 even when cultured pre-stimulated PDGF-BB. Preliminary results also indicate that stimulation dependent PDGFR-signaling and/or Additional studies ongoing vivo-like - invasion into brain slices being applied. this host factors explored "priming" factors, as TGF-beta PDGF-BB, inhibitors these pathways. co-culture- slice-assays will be used explore if cells, modulate sensitivity temozolomide commonly treatment.