9 – Purification, Fragmentation and Isotopic Labelling of Monoclonal Antibodies
作者： JAMES W. GODING
DOI: 10.1016/B978-012287023-1/50057-2
关键词: Chromatography 、 Polyclonal antibodies 、 Fragmentation (cell biology) 、 Antiserum 、 Labelling 、 Biology 、 Monoclonal antibody 、 Antibody
摘要: This chapter provides an overview of the purification, fragmentation, and isotopic labeling monoclonal antibodies. The physical properties antibodies vary somewhat among species, procedures that have been developed for a particular species do not always work well when applied to other species. is especially true attempts are made generate defined proteolytic fragments. purification designed polyclonal antisera optimized make reasonable compromise between yield purity. homogeneity makes them much easier purify; however, obtain good results, it advisable individualize conditions each antibody. methods described in proved reliable they usually involve minimal effort. descriptions antibody concentrate on IgG IgM because these classes most frequently encountered. common easiest purify. Most used either mouse or rat
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