Crosslinked polymeric self-assemblies as an efficient strategy for photodynamic therapy on a 3D cell culture

摘要: In order to compare the efficiency of crosslinked nano-vectors in field photodynamic therapy (PDT) both on 2D and 3D cell cultures, various polymeric self-assemblies based poly(ethyleneoxide-b-e-caprolactone) have been synthesized by radical polymerization acrylate end-functionalized polymers. Crosslinked obtained from reaction functionalized polymers with ethyleneglycoldimethacrylate (EGDMA) were compared chain-end polymerized unreacted ones. Polymeric micelles a size between 10 20 nm obtained, as well an elongated system length close 100 nm. They all characterized Transmission Electron Microscopy Dynamic Light Scattering but also Asymmetrical Flow Field-Flow Fractionation prove that they consisted pure self-assemblies. Chain-end or crosslinking did not induce any change morphology nor strong modification. After post-encapsulation photosensitizer, namely Pheophorbide a, systems examined for their potential use PDT HCT-116 FaDu human tumor lines cultures. The vectors observed be most efficient cultivated spheroids, whereas ones presented lower activity. This was different trend cultures where uncrosslinked micelle at concentration its analogue. allowed assessing influence polymer chain shape efficiency. molecular weight lead our case change, similar surface characteristics. As effect, self-assembly more than spherical micelles. are therefore promising treatment.