Design and Synthesis of Biotinylated Troglitazone as an Active Affinity Probe
作者： Xin Li , Xiaoqi Li , Hong Tang , Fajun Nan
关键词: Chemistry 、 In vitro 、 Biochemistry 、 Membrane protein 、 Troglitazone 、 Affinity chromatography 、 Apoptosis 、 Structure–activity relationship 、 Ligand (biochemistry) 、 Biotinylation 、 General chemistry
摘要: A biotin-tagged analogue of troglitazone was designed, synthesized and applied to affinity chromatography pulldown EGFR from 293T cell lysates overexpressing EGFR, a membrane protein assumed be troglitazone's direct binding target by previous work. The results indicate the feasibility biotinylated probe as vigorous tool clarify molecular mechanisms for antitumor activities.
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