Enhancement of Rayleigh Light Scattering of Acid Chrome Blue K by Proteins and Protein Assay by the Scattering Technique
作者： Chun Qi Ma , Ke An Li , Shen Yang Tong
DOI: 10.1039/A607359J
关键词: Aqueous solution 、 Linear range 、 Scattering 、 Fluorescence spectrometry 、 Chromatography 、 Fluorometer 、 Bradford protein assay 、 Metal ions in aqueous solution 、 Rayleigh scattering 、 Chemistry
摘要: Rayleigh light scattering of Acid Chrome Blue K (ACBK) is enhanced greatly by proteins. Based on this, a method for protein assay in aqueous solution was developed. This matches the sensitivity the colorimetric dye-binding with linear range of 0.136–10.88 µg ml - 1 . The measurements can be made easily common fluorimeter. reaction between ACBK and proteins is completed 2 min scattered signal stable at least 3 h. Protein-to-protein variability encountered this as many other assays. There little or no interference from amino acids, most metal ions complexing agents (e.g., EDTA). Interferences from salts, urea detergents minimized dilution.
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