Non-covalently functionalized single-walled carbon nanotube for topical siRNA delivery into melanoma.
作者： King Sun Siu , Di Chen , Xiufen Zheng , Xusheng Zhang , Nathan Johnston
DOI: 10.1016/J.BIOMATERIALS.2013.12.079
关键词: Small interfering RNA 、 MAPK/ERK pathway 、 Transdermal 、 RNA interference 、 Biophysics 、 Melanoma 、 Gene silencing 、 Materials science 、 Carbon nanotube 、 Gene expression 、 Molecular biology
摘要: RNAi can specifically regulate gene expression, but efficient delivery of siRNA in vivo is difficult while it has been shown that modified carbon nanotubes (CNT) protect siRNA, facilitate entry into cells and enhance transdermal drugs delivery. Single-walled (SWCNT) were functionalized non-covalently with succinated polyethyleimine (PEI-SA). In this study, the water soluble CNT, PEI-SA/CNT (IS/C) isolated characterized, silencing induced by IS/C/siRNA complexes was achieved in vitro in B16-F10 cells. In vivo topically applied to shaved mouse skin, as well a C57BL/6 mice melanoma model. We found significant uptake Cy3-labeled specific Braf (siBraf) tumor tissue. Treatment IS/C/siBraf resulted attenuation growth over 25-day period. This new method provided possibility for future therapy, which providing insight potential application development CNT-based
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