Evaluation of endopolyploidy patterns in selected Capsicum and Nicotiana species ( Solanaceae )

摘要: Endopolyploidy has arisen countless times in angiosperms, and endopolyploidisation is an important genetic feature many plant species. It generated through a process called endoreduplication, where mitotic cell cycle shifts into endocycle, DNA replication occurs without chromosome separation cytokinesis. been well established that endopolyploidy plays vital role during growth development various stress responses. Many agriculturally families are polysomatic, including the Solanaceae. To better understand characterise polysomatic species within Solanaceae, we studied mature vegetative reproductive organs (root, stem, lower leaf petiole, lamina, flower pedicel, calyx, corolla, pistil stamen tissue) of representative diploids genus Capsicum, i.e. C. annuum, baccatum, chinense diploid tetraploids Nicotiana, N. rustica (4x), sylvestris (2x) tabacum by means flow cytometry. The presence 2C–16C nuclei (rarely 32C) was detected, degree expressed using four different parameters for each organ analysed. In organs, endoreduplication index (EI) reached maximum 0.84 on average roots whereas lowest values (EI < 0.10) were detected lamina same Among investigated tabacum, EI pistils higher than stamens. When polyploid Nicotiana compared, possessed level polyploids tabacum. this study, also determined genome size species, which ranged from 5.51 picograms (pg) to 10.43 pg rustica.