Fatty acid-induced insulin resistance in adipocytes.
作者： Mark Van Epps-Fung , Jodie Williford , Alan Wells , Robert W. Hardy
关键词: Stimulation 、 Insulin 、 Glycogen 、 Glycogen synthase 、 In vivo 、 Endocrinology 、 Biology 、 Fatty acid 、 Internal medicine 、 Glucose transporter 、 Insulin resistance
摘要: Elevated serum-free fatty acid (FFA) levels induce insulin resistance in whole animals and humans. To understand the direct mechanism by which FFAs impact insulin-responsive tissue, we have used our previously developed vitro model of long-chain saturated acids (LCSFA)-induced adipocytes. In addition to explanted rat adipocytes, now demonstrate that overnight exposure 3T3-L1 adipocytes 1 mm individually LCSFA palmitate, myristate, stearate, leads an approximately 50% inhibition insulin-induced glucose transport. Insulin can be accomplished at 0.3 is within range palmitate found diabetic obese individuals. This was noted 4 h FFA, comparable vivo lipid infusion studies. Initial LCSFA-induced specific transport does not affect stimulation incorporation into glycogen. overexpressing EGF rec...
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