First comparative characterization of three distinct ferritin subunits from a teleost: Evidence for immune-responsive mRNA expression and iron depriving activity of seahorse (Hippocampus abdominalis) ferritins.

摘要: Ferritins play an indispensable role in iron homeostasis through their iron-withholding function living beings. In the current study, cDNA sequences of three distinct ferritin subunits, including a H, M, and L, were identified from big belly seahorse, Hippocampus abdominalis, molecularly characterized. Complete coding (CDS) seahorse H (HaFerH), M (HaFerM), L (HaFerL) subunits comprised 531, 528, 522 base pairs (bp), respectively, which encode polypeptides 177, 176, 174 amino acids, with molecular masses ∼20-21 kDa. Our silico analyses demonstrate that these exhibit typical characteristics superfamily members regulatory elements, domain signatures, reactive centers. The HaFerH, cloned corresponding proteins overexpressed bacterial system. Recombinantly expressed HaFer demonstrated detectable in vivo sequestrating (ferroxidase) activity, consistent putative binding capability. Quantification basal expression selected tissues gene-specific ubiquitous spatial distribution pattern, abundance mRNA HaFerM liver predominant HaFerH HaFerL blood. Interestingly, all genes was found to be significantly modulated against pathogenic stress mounted by lipopolysaccharides (LPS), poly I:C, Streptococcus iniae, Edwardsiella tarda. Collectively, our findings suggest may involved (II) they are important its host defense mechanisms.