Influence of commercial sanitizers on lipopolysaccharide production by Salmonella Enteritidis ATCC 13076.
作者： P. VENTER , M. ABRAHAM , J. F. R. LUES , I. IVANOV
DOI: 10.4315/0362-028X-69.12.2889
关键词: Biology 、 Bacteria 、 Toxicity 、 Salmonella enteritidis 、 Food microbiology 、 Hand sanitizer 、 Lipopolysaccharide 、 Enterobacteriaceae 、 Polysaccharide 、 Microbiology
摘要: The effect of typical sanitizers on the composition and toxicity lipopolysaccharides (LPSs) produced by Salmonella Enteritidis ATCC 13076 was analyzed. propagated up to late exponential phase in presence commercial sanitizing solutions. LPS extracted derivatized with trifluoroacetylation, gas chromatography-mass spectrometry analysis chromogenic Limulus amoebocyte lysate assay were used assess ultrastructure LPS. viability debris formation during growth evaluated verify bactericidal bacteriostatic effects sanitizer formation. LPSs quantified at 1.7 x 10(4), 1.2 3.6 10(3), 9.6 10(4) [KDO] OD(620nm)(-1) for controls organisms grown a chlorinated sanitizer, heavy-duty alkaline cleaner, phenolic hand wash solution, respectively. In response these treatments, short-chain polysaccharide fractions cells increased. This finding suggests that this organism increases low-molecular-weight fraction relation high-molecular-weight survive unfavorable conditions. cumulative change influenced LPS; however, could not be related an individual compound within any assessed fractions.
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jfoodprotection.com参考文章(45)
Michael E. St. Louis, The Emergence of Grade A Eggs as a Major Source of Salmonella enteritidis Infections JAMA. ,vol. 259, pp. 2103- 2107 ,(1988) , 10.1001/JAMA.1988.03720140023028
Craig Parker, Kokila Asokan, Jean Guard-Petter, Egg contamination by Salmonella serovar enteritidis following vaccination with Δ-aroA Salmonella serovar typhimurium Fems Microbiology Letters. ,vol. 195, pp. 73- 78 ,(2001) , 10.1111/J.1574-6968.2001.TB10500.X
M M Rahman, J Guard-Petter, R W Carlson, A virulent isolate of Salmonella enteritidis produces a Salmonella typhi-like lipopolysaccharide. Journal of bacteriology. ,vol. 179, pp. 2126- 2131 ,(1997) , 10.1128/JB.179.7.2126-2131.1997
Sirpa K Laitinen, Importance of sampling, extraction and preservation for the quantitation of biologically active endoto. Annals of Agricultural and Environmental Medicine. ,vol. 6, pp. 33- 38 ,(1999)
Jean Guard-Petter, Variants of Smooth Salmonella enterica Serovar Enteritidis That Grow to Higher Cell Density Than the Wild Type Are More Virulent Applied and Environmental Microbiology. ,vol. 64, pp. 2166- 2172 ,(1998) , 10.1128/AEM.64.6.2166-2172.1998
J Guard-Petter, B Lakshmi, R Carlson, K Ingram, Characterization of lipopolysaccharide heterogeneity in Salmonella enteritidis by an improved gel electrophoresis method. Applied and Environmental Microbiology. ,vol. 61, pp. 2845- 2851 ,(1995) , 10.1128/AEM.61.8.2845-2851.1995
Henrik C Wegener, Tine Hald, Lo Fo Wong, Mogens Madsen, Helle Korsgaard, Flemming Bager, Peter Gerner-Smidt, Kåre Mølbak, None, Salmonella control programs in Denmark. Emerging Infectious Diseases. ,vol. 9, pp. 774- 780 ,(2003) , 10.3201/EID0907.030024
Ernst T. Rietschel, Teruo Kirikae, F. Ulrich Schade, Uwe Mamat, Günter Schmidt, Harald Loppnow, Artur J. Ulmer, Ulrich Zähringer, Ulrich Seydel, Franco Di Padova, Max Schreier, Helmut Brade, Bacterial endotoxin: molecular relationships of structure to activity and function. The FASEB Journal. ,vol. 8, pp. 217- 225 ,(1994) , 10.1096/FASEBJ.8.2.8119492
J. Guard-Petter, L. H. Keller, M. Mahbubur Rahman, R. W. Carlson, S. Silvers, A novel relationship between O-antigen variation, matrix formation, and invasiveness of Salmonella enteritidis. Epidemiology and Infection. ,vol. 117, pp. 219- 231 ,(1996) , 10.1017/S0950268800001394
Craig D. Ellermeier, James M. Slauch, The Genus Salmonella Springer New York. pp. 123- 158 ,(2006) , 10.1007/0-387-30746-X_7