The effect of temperature on candidate gene expression in the brain of honey bee Apis mellifera (Hymenoptera: Apidae) workers exposed to neonicotinoid imidacloprid.

摘要: Neonicotinoid insecticides are potent agonists of nicotinic acetylcholine receptors and a major factor in the decline pollinators worldwide. Several studies show that low doses this neurotoxin influence honey bee physiology, however, little is known about how interact with other environmental variables. We studied effects two neonicotinoid Imidacloprid (IMD, 0, 2.5, 10 ppb), three temperatures (20, 28, 36°C) on gene expression brains worker bees (Apis mellifera). Using qRT-PCR we quantified eight key genes related to nervous system, stress response, motor olfactory capacities. Gene tended increase IMD dose, which was further intensified individuals maintained cold treatment (20°C). At 20°C octopamine receptor (oa1) underexpressed were not exposed IMD, but overexpressed 2.5 ppb IMD. Also, heat shock proteins (hsp70 hsp90) increased their at high (36°C), doses. These results suggest despite insecticide concentrations used study (a field-realistic dose), changes associated physiological responses could be induced. This contributes understanding residual may alter physiology.