MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate

摘要: Results and Discussion Two MR contrast agents, conjugated to PNA cell penetrating peptide, were synthesized characterized by ESI-MS. Fluorescence studies showed that both agents could enter efficiently into 3T3 cells in a concentration dependent manner from 0.5 M 9.0 M; the agent was located vesicles around nucleus. The results of vitro statistically significant increase intracellular relaxation rate R1,cell already at labeling (Fig.1, Table 1), thus enhancement also detectable images (Fig.2). R2,cell less prominent. A preliminary binding assay with an immobilized target oligodeoxynucleotide sequence higher specificity antisense CA comparison its nonsense counterpart. 1. Intracellular rates after loading dsRed for 18 h.