The mouse antibody response to Trichinella spiralis defines a single, immunodominant epitope shared by multiple antigens.

摘要: Immunoblot analysis was used to characterize the Trichinella spiralis L1 larval Ag recognized by antisera from T. spiralis-infected AKR/J mice. Antisera were analyzed for reactivity with crude worm extract, excretory/secretory proteins and cuticle larvae. The response biphasic; antibodies against one set of detected 13 days after infection (group I Ag), a different 35 II Ag). Excretory/secretory only produced late in infection. predominant isotype day 42 antiserum IgG1, 80% these IgG1 reacted stage-specific determinant shared virtually all group Ag. A mAb reactive purify extract. Such affinity-purified protective when immunize mice subsequent infection, T cells infected proliferated cultured vitro. Other mouse strains also made strong serum antibody We hypothesize that immune responses epitope play an important role determining outcome host-parasite interaction during