EFFECTS OF MYCOTOXINS ON CYTOKINE PRODUCTION AND PROLIFERATION IN EL-4 THYMOMA CELLS

摘要: The thymoma cell line EL4.IL-2 (EL-4) was used as a T-cell model to assess the immunotoxic effects of several mycotoxins produced by Aspergillus-Penicillium and Fusarium groups. EL-4 cells were stimulated with phorbol 12-myristate 12-acetate (PMA) in presence at various concentrations for 5 d culture supernatants analyzed interleukins (IL) IL-2 IL-5 enzyme-linked immunosorbent assay (ELISA). cytokine further related proliferation viability using MTT [3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide] absorbance 570 nm (A570) endpoint indicator. levels dramatically increased cyclopiazonic acid 50-1000 ng/ml, whereas significantly decreased 10 microgram/ml. Proliferation slightly 100-1000 ng/ml but markedly depressed When exposed microgram/ml ochratoxin A, production while decreased. A570 A Il-5 almost totally suppressed patulin > or = 500 T-2 toxin ng/ml. These occurred concurrently marked depression assay. Although unaffected either zearalenone alpha-zearalenol exposure, both elevated these toxins not affected cultured aflatoxin B1 secalonic wortmannin, fumonisin B1, fusaric up In total, studies indicated that acid, zearalenone, could stimulate inhibitory. Cytokine dysregulation always directly perturbations proliferation. results suggest be simple effective vitro evaluating immunotoxicity classes environmental chemicals.