Rapamycin prevents the onset of insulin-dependent diabetes mellitus (IDDM) in NOD mice.

摘要: The effect of the immunosuppressive agent rapamycin (RAPA) was assessed in non-obese diabetic (NOD) mouse which is an autoimmune model IDDM. RAPA prepared a vehicle 8% cremophor EL/2% ethanol and investigated two studies. NOD/MrK female mice (six per group, study no. 1; 10 2) were dosed three times week p.o. by gavage from 56 to 170 days age (study 1) or 64 176 2). Mice treated with at 0.6 mg/kg, 6 12 mg/kg maintained normal plasma glucose through 10%, 0%, 0% incidence diabetes respectively. In contrast, naive, vehicle-treated, 0.06 mg/kg-treated exhibited elevated disease typical for NOD mice. became had levels beta-hydroxybutyrate, triglycerides cholesterol. These lipid concentrations positively correlated duration hyperglycaemia (r = 0.85, 0.87 0.84 respectively). Outside its ability prevent diabetes, itself did not affect profile Intervention therapy ineffective reversing course after IDDM onset under these experimental conditions. Finally, we report here that prophylactic treatment able protect against development some RAPA-treated 41 weeks cessation treatment. data show orally administered effective preventing mouse, relevant type I man.