Cloning and mapping of the manganese superoxide dismutase gene (sodA) of Escherichia coli K-12.

摘要: An Escherichia coli gene bank composed of large DNA fragments (about 40 kilobases) was constructed by using the small cosmid pHC79. From it, a clone isolated for its ability to overproduce superoxide dismutase. The enzyme overproduced manganese dismutase, as determined electrophoresis and antibody precipitation. Maxicell analysis two-dimensional O'Farrell polyacrylamide gel demonstrated that structural gene, sodA, dismutase cloned. Subcloning from original located sodA within 4.8-kilobase EcoRI-BamHI fragment. This fragment inserted into lambda phage which deleted att region consequently could only lysogenize recombination between cloned bacterial insertion chromosome. Genetic mapping prophage in such lysogens indicated chromosomal locus lies near 87 min on E. map.